Optimization of Multiplication and Initiation Media for Anthurium Maine using Growth Regulators

Abstract

This research optimized the initiation and multiplication media for Anthurium Maine using plant growth regulators. The study evaluated the effects of different concentrations of 2, 4-D, and kinetin on callus growth using leaf explants for initiation and established callus for multiplication. It was found, that in initiation, T1 achieves the highest callus weight, especially under dark conditions. For multiplication, T1 produced the best results for callus weight and callus diameter. The study highlights the importance of low Auxin and cytokinin concentrations, along with dark conditions, in optimizing Anthurium tissue culture for large-scale propagation.